- Endocrine Research
- Transformation of Mature Osteoblasts into Bone Lining Cells and RNA Sequencing-Based Transcriptome Profiling of Mouse Bone during Mechanical Unloading
-
A Ram Hong, Kwangsoo Kim, Ji Yeon Lee, Jae-Yeon Yang, Jung Hee Kim, Chan Soo Shin, Sang Wan Kim
-
Endocrinol Metab. 2020;35(2):456-469. Published online June 24, 2020
-
DOI: https://doi.org/10.3803/EnM.2020.35.2.456
-
Correction in: Endocrinol Metab 2021;36(6):1314
-
7,639
View
-
172
Download
-
10
Web of Science
-
10
Crossref
-
Abstract
PDFSupplementary MaterialPubReader ePub
- Background
We investigated RNA sequencing-based transcriptome profiling and the transformation of mature osteoblasts into bone lining cells (BLCs) through a lineage tracing study to better understand the effect of mechanical unloading on bone loss.
Methods Dmp1-CreERt2(+):Rosa26R mice were injected with 1 mg of 4-hydroxy-tamoxifen three times a week starting at postnatal week 7, and subjected to a combination of botulinum toxin injection with left hindlimb tenotomy starting at postnatal week 8 to 10. The animals were euthanized at postnatal weeks 8, 9, 10, and 12. We quantified the number and thickness of X-gal(+) cells on the periosteum of the right and left femoral bones at each time point.
Results Two weeks after unloading, a significant decrease in the number and a subtle change in the thickness of X-gal(+) cells were observed in the left hindlimbs compared with the right hindlimbs. At 4 weeks after unloading, the decrease in the thickness was accelerated in the left hindlimbs, although the number of labeled cells was comparable. RNA sequencing analysis showed downregulation of 315 genes in the left hindlimbs at 2 and 4 weeks after unloading. Of these, Xirp2, AMPD1, Mettl11b, NEXN, CYP2E1, Bche, Ppp1r3c, Tceal7, and Gadl1 were upregulated during osteoblastogenic/osteocytic and myogenic differentiation in vitro.
Conclusion These findings demonstrate that mechanical unloading can accelerate the transformation of mature osteoblasts into BLCs in the early stages of bone loss in vivo. Furthermore, some of the genes involved in this process may have a pleiotropic effect on both bone and muscle.
-
Citations
Citations to this article as recorded by
- Insights and implications of sexual dimorphism in osteoporosis
Yuan-Yuan Zhang, Na Xie, Xiao-Dong Sun, Edouard C. Nice, Yih-Cherng Liou, Canhua Huang, Huili Zhu, Zhisen Shen Bone Research.2024;[Epub] CrossRef - METTLing in Stem Cell and Cancer Biology
John G. Tooley, James P. Catlin, Christine E. Schaner Tooley Stem Cell Reviews and Reports.2023; 19(1): 76. CrossRef - Three's a crowd – why did three N-terminal methyltransferases evolve for one job?
Meghan M. Conner, Christine E. Schaner Tooley Journal of Cell Science.2023;[Epub] CrossRef - Unlocking the mysteries of alpha-N-terminal methylation and its diverse regulatory functions
Panyue Chen, Rong Huang, Tony R. Hazbun Journal of Biological Chemistry.2023; 299(7): 104843. CrossRef - Examining Mechanisms for Voltage-Sensitive Calcium Channel-Mediated Secretion Events in Bone Cells
Perla C. Reyes Fernandez, Christian S. Wright, Mary C. Farach-Carson, William R. Thompson Calcified Tissue International.2023; 113(1): 126. CrossRef - Reactivation of Bone Lining Cells are Attenuated Over Repeated Anti-sclerostin Antibody Administration
A Ram Hong, Jae-Yeon Yang, Ji Yeon Lee, Joonho Suh, Yun-Sil Lee, Jung-Eun Kim, Sang Wan Kim Calcified Tissue International.2022; 111(5): 495. CrossRef - Purine metabolism in the development of osteoporosis
Keda Yang, Jie Li, Lin Tao Biomedicine & Pharmacotherapy.2022; 155: 113784. CrossRef - Effects of Gabapentin and Pregabalin on Calcium Homeostasis: Implications for Physical Rehabilitation of Musculoskeletal Tissues
Perla C. Reyes Fernandez, Christian S. Wright, Stuart J. Warden, Julia Hum, Mary C. Farach-Carson, William R. Thompson Current Osteoporosis Reports.2022; 20(6): 365. CrossRef - Calvaria Bone Transcriptome in Mouse Models of Osteogenesis Imperfecta
Pierre Moffatt, Iris Boraschi-Diaz, Juliana Marulanda, Ghalib Bardai, Frank Rauch International Journal of Molecular Sciences.2021; 22(10): 5290. CrossRef - Age-related neurodegeneration and cognitive impairments of NRMT1 knockout mice are preceded by misregulation of RB and abnormal neural stem cell development
James P. Catlin, Leandro N. Marziali, Benjamin Rein, Zhen Yan, M. Laura Feltri, Christine E. Schaner Tooley Cell Death & Disease.2021;[Epub] CrossRef
|